No clinical characteristics related to the HPV status of the tumors were observed in our series. Basaloid carcinoma is also frequently associated with HPV genomes and is more likely to represent a histologic variant of squamous cell carcinoma than a separate entity. An analysis of these data within the framework of the literature indicates that approximately 75% of cases of invasive anal carcinoma can actually be considered associated with HPV sequences. The carcinoma in situ case and the Paget's disease case were negative. Histovirologic correlations showed that 12 of 18 squamous cell carcinomas were associated with HPV 16 and that 8 of 9 cases of basaloid carcinoma were HPV positive 5 case corresponded to HPV 16, 2 cases to HPV 18, and 1 case to HPV X. HPV 16 DNA was found in 17 cases, HPV 18 in 2 cases, and in 1 case, the type of the HPV sequences detected remained undetermined (HPV X). In our series, 20 (74%) of 27 cases of invasive anal carcinoma were positive for HPV DNA by Southern blot hybridization and/or polymerase chain reaction. We have looked for correlations between virologic data and histologic types of tumors, and analyzed the clinical characteristics of HPV-positive and HPV-negative lesions. By use of both polymerase chain reaction and Southern blot hybridization on DNA extracted from frozen tissue specimens, we looked for HPV sequences in 12 cases of squamous cell carcinoma, in 9 cases of basaloid invasive carcinoma, in 1 case of carcinoma in situ, and in 1 case of Paget's disease of the anal canal. Moreover, the relationship of HPV to basaloid carcinoma, claimed to represent a histologic type different from the common squamous cell carcinoma, is still controversial. Their role in anal carcinogenesis is less clear, and the rate of HPV genome detected in invasive anal carcinoma varies considerably in the different reports. Human papillomaviruses (HPVs) play a major role in the development of genital neoplasia. The detection of HPV DNA may be useful in distinguishing primary from metastatic adenoCA of the cervix. We conclude that HPV 16/18 DNA is present in a significant proportion of primary adenoCA of the cervix, and we have identified some clinicopathologic associations.
HPV 16/18 DNA was not detected in six metastatic adenoCA to cervix (four endometrial, two ovarian). The presence of squamous carcinoma in situ, koilocytosis and younger patient age were associated with an increased incidence of HPV 16/18 DNA detection. In three patients, two different specimens were evaluated, and there was concordance of HPV typing. There were no tumors with mixed infections. HPV 18 DNA was identified in 13/22 (59%) and HPV 16 DNA was identified in 5/22 (23%) of the tumors. The amplification products were analyzed using gel electrophoresis and Southern dot blotting with 32p labeled type-specific oligonucleotide probes. PCR was used to specifically amplify the E6-E7 gene region of HPV 16/18 DNA. Note : This information is taken from the Journal Citation Reports™ (Clarivate).Twenty-two tissue samples of primary adenocarcinoma (adenoCA) of the uterine cervix were evaluated for the presence of HPV 16/18 DNA using the polymerase chain reaction (PCR). In other words, we can say that the higher the impact factor, the higher the rank of a journal. But the impact factor works beyond this and shows that numbers are not absolute. Well, we all know that watch the numbers and higher will win. On the other hand, along with the impact factor, it is important to know about the functional impact factor. The definition of impact factor is the number of citations, the articles published in that journal during the last two preceding years, in the given year and divide this by the total number of citable items, which published in that journal in the previous two years. Moreover, impact factor is having all the information which is published in the last two years in the given journal received.īefore moving forward, it is essential to know about the necessary information about the impact factor. It is based on the scientometric index that shows the annual average number of citations. The Impact factor is also known by the name of journal impact factor of an academic journal.
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